Multicolor re-scan super-resolution imaging of live cells
نویسندگان
چکیده
منابع مشابه
Multicolor super-resolution DNA imaging for genetic analysis.
Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled ...
متن کاملMulticolor Super-Resolution Fluorescence Imaging via Multi-Parameter Fluorophore Detection
Understanding the complexity of the cellular environment will benefit from the ability to unambiguously resolve multiple cellular components, simultaneously and with nanometer-scale spatial resolution. Multicolor super-resolution fluorescence microscopy techniques have been developed to achieve this goal, yet challenges remain in terms of the number of targets that can be simultaneously imaged ...
متن کاملMulticolor super-resolution imaging with photo-switchable fluorescent probes.
Recent advances in far-field optical nanoscopy have enabled fluorescence imaging with a spatial resolution of 20 to 50 nanometers. Multicolor super-resolution imaging, however, remains a challenging task. Here, we introduce a family of photo-switchable fluorescent probes and demonstrate multicolor stochastic optical reconstruction microscopy (STORM). Each probe consists of a photo-switchable "r...
متن کاملCytokinesis: Going Super-Resolution in Live Cells
Super-resolution fluorescence microscopy has emerged as a powerful tool for studying molecular organization, but mostly in fixed cells. New work using high-speed fluorescence photoactivation localization microscopy now reveals the organization of cytokinesis nodes and contractile rings in live fission yeast cells.
متن کاملImaging HIV-1 RNA dimerization in cells by multicolor super-resolution and fluctuation microscopies
Dimerization is a unique and vital characteristic of retroviral genomes. It is commonly accepted that genomic RNA (gRNA) must be dimeric at the plasma membrane of the infected cells to be packaged during virus assembly. However, where, when and how HIV-1 gRNA find each other and dimerize in the cell are long-standing questions that cannot be answered using conventional approaches. Here, we comb...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Quantitative Imaging in Medicine and Surgery
سال: 2019
ISSN: 2223-4292,2223-4306
DOI: 10.21037/qims.2019.05.05